UJI EFEKTIVITAS AIR REBUSAN DAUN SIRIH (Piper betle) DAN DAUN TEH (Camellia sinensis) SEBAGAI ANTIMIKROBA BAKTERI Eschericia coli DALAM UPAYA PENYUSUNAN PETUNJUK PRAKTIKUM MIKROBIOLOGI
Keywords:Betel Leaf Decoction, Tea Leaf Decoction, Escherichia coli, Bacteria
Boiled water is a traditional medicinal drink used by Indonesian people since ancient times, one of the plants whose leaves can be used to make boiled water is betel leaves and tea leaves as a medicine for diarrheal diseases. This is a descriptive research and only proves antibacterial activity by measuring the diameter of the inhibition zone for the activity of betel leaf and tea leaf decoction in inhibiting the growth process of Escherichia coli bacteria. The method used is the diffusion method. The concentrations used in this study were 25%, 50%, 75% and 100%. The negative control used was distilled water and the positive control used was amoxicillin. A decoction with a concentration variation of 75% forms the largest inhibition zone because at this concentration it is able to maximally attract the polar active compounds that are owned so that they can work optimally in inhibiting bacterial growth. The betel leaf decoction formed an average inhibition zone of 2.8 mm, secondly the tea leaf stew formed an average inhibition zone of 2.1 mm and a combination of betel leaves and tea leaves formed an inhibition zone with an average of 3.5mm. The results of the feasibility test of the microbiology practicum manual that have been analyzed obtained the validation results of material experts of 95%, media experts of 95%, and language experts of 85% and from these results the microbiology practicum manual is feasible to use.
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